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Sample GSM1223993 Query DataSets for GSM1223993
Status Public on Jan 16, 2014
Title P. mallei monoclonal P. mallei_1
Sample type protein
 
Source name P. mallei
Organism Mus musculus
Characteristics antibody: P. mallei monoclonal
Extracted molecule protein
Extraction protocol NA
Label biotinylated anti-mouse IgG
Label protocol Alexafluor 647-labeled Streptavidin was used to detect biotinylated anti-mouse secondary antibody.
 
Hybridization protocol Antibodies were incubated with the peptide microarrays for 1 hour at room temperature according to Stafford et al. (Stafford, Halperin, Legutki, Magee, Galgiani, Johnston, Physical characterization of the Immunosignaturing Effect, MCP 11(4), 2012).
Scan protocol Scanning was done on an Innopsys Innoscan 910 at 10um resolution, high laser power, 70% PMT, autofocus ON, 545nm.
Description Alexa-647 conjugated Streptavidin tertiary
P. mallei_1
Data processing Median normalization per slide was done, and any negative values were adjusted to 0.01.
 
Submission date Sep 04, 2013
Last update date Jan 16, 2014
Contact name Phillip Stafford
E-mail(s) Phillip.Stafford@asu.edu
Organization name Arizona State University
Department Biodesign Institute
Lab Center for Innovations in Medicine
Street address 727 E. Tyler St.
City Tempe
State/province Arizona
ZIP/Postal code 85287
Country USA
 
Platform ID GPL17490
Series (2)
GSE49217 In situ synthesis of peptide microarrays - shadow mask design [1]
GSE50045 In situ synthesis of peptide microarrays - shadow mask design

Data table header descriptions
ID_REF
VALUE GeneSpring 7.3.1 was used to load and median-normalize raw gpr files. Matrix of values was copied directly from GeneSpring into Excel as double-precision values.

Data table
ID_REF VALUE
1 1.14609375
2 1.12890625
3 1.06796875
4 1.04453125
5 1.06796875
6 1.096875
7 1.12421875
8 1.18359375
9 1.1859375
10 1.0625
11 0.97578125
12 1.003125
13 0.9703125
14 0.90546875
15 0.7875
16 0.79609375
17 0.84375
18 0.84375
19 0.86328125
20 0.925

Total number of rows: 1536

Table truncated, full table size 21 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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