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Sample GSM1223983 Query DataSets for GSM1223983
Status Public on Jan 16, 2014
Title LNKB2 monoclonal LNKB2_1
Sample type protein
 
Source name LNKB2
Organism Mus musculus
Characteristics antibody: LNKB2 monoclonal
Extracted molecule protein
Extraction protocol NA
Label biotinylated anti-mouse IgG
Label protocol Alexafluor 647-labeled Streptavidin was used to detect biotinylated anti-mouse secondary antibody.
 
Hybridization protocol Antibodies were incubated with the peptide microarrays for 1 hour at room temperature according to Stafford et al. (Stafford, Halperin, Legutki, Magee, Galgiani, Johnston, Physical characterization of the Immunosignaturing Effect, MCP 11(4), 2012).
Scan protocol Scanning was done on an Innopsys Innoscan 910 at 10um resolution, high laser power, 70% PMT, autofocus ON, 545nm.
Description Alexa-647 conjugated Streptavidin tertiary
LNKB2_1
Data processing Median normalization per slide was done, and any negative values were adjusted to 0.01.
 
Submission date Sep 04, 2013
Last update date Jan 16, 2014
Contact name Phillip Stafford
E-mail(s) Phillip.Stafford@asu.edu
Organization name Arizona State University
Department Biodesign Institute
Lab Center for Innovations in Medicine
Street address 727 E. Tyler St.
City Tempe
State/province Arizona
ZIP/Postal code 85287
Country USA
 
Platform ID GPL17490
Series (2)
GSE49217 In situ synthesis of peptide microarrays - shadow mask design [1]
GSE50045 In situ synthesis of peptide microarrays - shadow mask design

Data table header descriptions
ID_REF
VALUE GeneSpring 7.3.1 was used to load and median-normalize raw gpr files. Matrix of values was copied directly from GeneSpring into Excel as double-precision values.

Data table
ID_REF VALUE
1 1.304458599
2 0.662420382
3 1.620382166
4 1.087898089
5 1.373248408
6 1.049681529
7 1.352866242
8 0.998726115
9 1.049681529
10 0.626751592
11 1.207643312
12 1.052229299
13 0.573248408
14 0.996178344
15 1.207643312
16 1.645859873
17 0.932484076
18 0.792356688
19 1.05477707
20 1.406369427

Total number of rows: 1536

Table truncated, full table size 24 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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