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Sample GSM1220298

Query DataSets for GSM1220298

Status

Public on Jan 29, 2014

Title

Reference Epigenome: ChIP-Seq Analysis of H3K4me3 in Human Liver Tissue; renlab.H3K4me3.STL011LI.01.01

Sample type

SRA

Source name

Liver tissue; renlab.H3K4me3.STL011LI.01.01

Organism

Homo sapiens

Characteristics

sample alias: STL011LI-01
disease: coronary artery disease
biomaterial_provider: Shin Lin, Stanford University
biomaterial_type: Primary Tissue
tissue_type: Liver
tissue_depot: N/A
collection_method: Autopsy
donor_id: STL011
donor_age: 32
donor_health_status: coronary artery disease. No history of hypertension or diabetes.
donor_sex: Male
donor_ethnicity: Caucasian
experiment_type: Histone H3K4me3
extraction_protocol: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
extraction_protocol_type_of_sonicator: Biorupter
extraction_protocol_sonication_cycles: 80
chip_protocol: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
chip_protocol_chromatin_amount: 500 micrograms
chip_protocol_bead_type: magnetic anti-rabbit
chip_protocol_bead_amount: 33,500,000
chip_protocol_antibody_amount: 5 micrograms
chip_antibody: H3K4me3
chip_antibody_provider: Millipore
chip_antibody_catalog: 04-745
chip_antibody_lot: 2197316

Extracted molecule

genomic DNA

Extraction protocol

Library construction protocol: See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf

Library strategy

ChIP-Seq

Library source

genomic

Library selection

ChIP

Instrument model

Illumina HiSeq 2500