|
| Status |
Public on Jul 19, 2007 |
| Title |
370 v 10R #1 Cy5Cy3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
M1 SF370
|
| Organism |
Streptococcus pyogenes |
| Characteristics |
M1T1 SF370
|
| Biomaterial provider |
Temekka Leday
|
| Growth protocol |
37C in closed cap tubes to late exponential phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CsCl isolation with ultracentrifugation
|
| Label |
Cy5
|
| Label protocol |
Amersham CyScribe Post-Labelling Kit
|
| |
|
| Channel 2 |
| Source name |
M1 SF370.10R
|
| Organism |
Streptococcus pyogenes |
| Characteristics |
M1T1 SF370 (spt10R-)
|
| Biomaterial provider |
Temekka Leday
|
| Growth protocol |
37C in closed cap tubes to late exponential phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CsCl isolation with ultracentrifugation
|
| Label |
Cy3
|
| Label protocol |
Amersham CyScribe Post-Labelling Kit
|
| |
|
| |
| Hybridization protocol |
50C overnight
|
| Scan protocol |
GenePix 6.1
|
| Description |
Details located in Ribardo et al 2006
|
| Data processing |
ratio-based Pearson Centered normalization using Acuity 4.0
|
| |
|
| Submission date |
Jul 19, 2006 |
| Last update date |
Jul 24, 2006 |
| Contact name |
Kevin S McIver |
| E-mail(s) |
kevin.mciver@umd.edu
|
| Phone |
301-405-4136
|
| Fax |
301-314-9082
|
| Organization name |
University of Maryland, College Park
|
| Department |
Cell Biology and Molecular Genetics
|
| Street address |
3124 Biociences Research Bldg. (413)
|
| City |
College Park |
| State/province |
MD |
| ZIP/Postal code |
20742 |
| Country |
USA |
| |
|
| Platform ID |
GPL1482 |
| Series (1) |
|
