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Sample GSM1208310 Query DataSets for GSM1208310
Status Public on Aug 15, 2013
Title o- organoid
Sample type RNA
Source name crypt-villus organoid
Organism Mus musculus
Characteristics strain: C57/BL6
Extracted molecule total RNA
Extraction protocol AGPC metnod for preparation of RNA extraction
Label Cy3
Label protocol 40 ng total RNA from sample o- organoid and 20 ng total RNA from all other samples was used as template to produce Cy3- labeled cRNA. The RNA samples were amplified and labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer’s protocol.
Hybridization protocol Cy3 labeled cRNAs were hybridized overnight (17 hours, 65°C) to an Agilent Whole Mouse Genome Oligo Microarrays (4x44K V2) using Agilent’s recommended hybridization chamber and oven.Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
Description wild tyle
Data processing The Agilent Feature Extraction Software (FES ) was used to read out and process the microarray image files.
Submission date Aug 14, 2013
Last update date Aug 15, 2013
Contact name Toshio Takahashi
Organization name Suntory Foundation for Life Sciences
Street address 1-1-1 Wakayamadai
City Osaka
ZIP/Postal code 618-8503
Country Japan
Platform ID GPL10333
Series (1)
GSE49864 Non-neuronal acetylcholine as an endogenous regulator of proliferation and differentiation of Lgr5-positive stem cells in mice

Data table header descriptions
VALUE median normalized signal intensities

Data table
12 0.157407
13 0.156875
14 0.15638
15 0.155846
16 51.428513
17 91.555303
18 14.882634
19 0.154105
20 0.153724
21 0.294472
22 3.627824
23 347.301832
24 149.629798
25 11.293239
26 0.151894
27 98.586608
28 129.823624
30 69.157783
31 121.141078
32 0.15075

Total number of rows: 43020

Table truncated, full table size 633 Kbytes.

Supplementary file Size Download File type/resource
GSM1208310_252665511299_S01_GE1_107_Sep09_1_3.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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