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Sample GSM1208309 Query DataSets for GSM1208309
Status Public on Aug 15, 2013
Title y- organoid
Sample type RNA
 
Source name crypt-villus organoid
Organism Mus musculus
Characteristics strain: C57/BL6
Extracted molecule total RNA
Extraction protocol AGPC metnod for preparation of RNA extraction
Label Cy3
Label protocol 40 ng total RNA from sample o- organoid and 20 ng total RNA from all other samples was used as template to produce Cy3- labeled cRNA. The RNA samples were amplified and labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer’s protocol.
 
Hybridization protocol Cy3 labeled cRNAs were hybridized overnight (17 hours, 65°C) to an Agilent Whole Mouse Genome Oligo Microarrays (4x44K V2) using Agilent’s recommended hybridization chamber and oven.Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
Description wild tyle
Data processing The Agilent Feature Extraction Software (FES 10.7.3.1 ) was used to read out and process the microarray image files.
 
Submission date Aug 14, 2013
Last update date Aug 15, 2013
Contact name Toshio Takahashi
E-mail(s) takahashi@sunbor.or.jp
Organization name Suntory Foundation for Life Sciences
Street address 1-1-1 Wakayamadai
City Osaka
ZIP/Postal code 618-8503
Country Japan
 
Platform ID GPL10333
Series (1)
GSE49864 Non-neuronal acetylcholine as an endogenous regulator of proliferation and differentiation of Lgr5-positive stem cells in mice

Data table header descriptions
ID_REF
VALUE median normalized signal intensities

Data table
ID_REF VALUE
12 0.160409
13 0.160342
14 0.160272
15 0.160309
16 21.836371
17 22.627813
18 2.874393
19 0.160559
20 0.160714
21 0.160863
22 1.632377
23 706.31622
24 56.146046
25 6.034772
26 0.162308
27 70.854401
28 74.837706
30 26.58171
31 48.827898
32 0.165116

Total number of rows: 43020

Table truncated, full table size 630 Kbytes.




Supplementary file Size Download File type/resource
GSM1208309_252665511299_S01_GE1_107_Sep09_1_2.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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