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Sample GSM1208308 Query DataSets for GSM1208308
Status Public on Aug 15, 2013
Title intestine
Sample type RNA
Source name whole intestine
Organism Mus musculus
Characteristics strain: C57/BL6
Extracted molecule total RNA
Extraction protocol AGPC metnod for preparation of RNA extraction
Label Cy3
Label protocol 40 ng total RNA from sample o- organoid and 20 ng total RNA from all other samples was used as template to produce Cy3- labeled cRNA. The RNA samples were amplified and labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer’s protocol.
Hybridization protocol Cy3 labeled cRNAs were hybridized overnight (17 hours, 65°C) to an Agilent Whole Mouse Genome Oligo Microarrays (4x44K V2) using Agilent’s recommended hybridization chamber and oven.Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
Description wild tyle
Data processing The Agilent Feature Extraction Software (FES ) was used to read out and process the microarray image files.
Submission date Aug 14, 2013
Last update date Aug 15, 2013
Contact name Toshio Takahashi
Organization name Suntory Foundation for Life Sciences
Street address 1-1-1 Wakayamadai
City Osaka
ZIP/Postal code 618-8503
Country Japan
Platform ID GPL10333
Series (1)
GSE49864 Non-neuronal acetylcholine as an endogenous regulator of proliferation and differentiation of Lgr5-positive stem cells in mice

Data table header descriptions
VALUE median normalized signal intensities

Data table
12 0.745594
13 0.114355
14 13.557127
15 0.683765
16 23.401591
17 62.927171
18 6.972289
19 0.114289
20 1.765923
21 2.024088
22 1.631122
23 385.462715
24 34.048528
25 4.23976
26 0.114891
27 29.76171
28 446.15458
30 52.138269
31 80.79667
32 0.115942

Total number of rows: 43020

Table truncated, full table size 629 Kbytes.

Supplementary file Size Download File type/resource
GSM1208308_252665511299_S01_GE1_107_Sep09_1_1.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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