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Sample GSM1206684 Query DataSets for GSM1206684
Status Public on Dec 02, 2013
Title xHomo-3
Sample type SRA
Source name xHomo_E9.5 embryo
Organism Mus musculus
Characteristics strain background: C57BL/6J
genotype/variation: xHomo
developmental stage: E9.5
tissue: embryo
Extracted molecule total RNA
Extraction protocol Total RNA was purified with an RNeasy Mini Kit (QIAGEN), in which an on-column DNase treatment was included.
500 ng of purified RNA was then subjected to mRNA isolation and library preparation using a NEBNext Ultra RNA Directional Library Prep Kit for Illumina with the Poly (A) mRNA Magnetic Isolation Module (NEB) following manufacturer’s instructions.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Data processing Illumina Bcl2fastq 1.8.2 software was used for demultiplexing and generation of fastq files
RNA-seq data were processed with Tophat and Cufflinks using the UCSC mm9 annotation
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample
Submission date Aug 11, 2013
Last update date May 15, 2019
Contact name Li Shen
Organization name HHMI/Boston Children's Hospital/Harvard Medical School
Street address 200 Longwood Ave
City Boston
ZIP/Postal code 02115
Country USA
Platform ID GPL17021
Series (2)
GSE49761 Role of Tet1 in genomic imprinting erasure [RNA-seq]
GSE49764 Role of Tet1 in genomic imprinting erasure
BioSample SAMN02315353
SRA SRX333422

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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