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Sample GSM1206263 Query DataSets for GSM1206263
Status Public on Nov 16, 2013
Title m12_b4l1 Bisulfite-Seq
Sample type SRA
 
Source name 12 month Hematopoietic Stem Cells
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Mx1-cre-;Dnmt3afl/fl
cell type: Murine bone marrow derived primary HSCs
cell surface marker: SP-KSL-CD150+
Treatment protocol Adapter-ligated fragments were treated with sodium bisulfite (Qiagen)
Growth protocol Freshly sorted 12 month wild type (Mx1-cre-;Dnmt3afl/fl) and knockout (Mx1-cre+;Dnmt3a-/-) mouse HSCs (SP-KSL-CD150+)
Extracted molecule genomic DNA
Extraction protocol Extract genomic DNA using Invitrogen PureLink Genomic DNA kits
Tru-Seq DNA sample prep kit
 
Library strategy Bisulfite-Seq
Library source genomic
Library selection RANDOM
Instrument model Illumina HiSeq 2000
 
Description technical replicate of biological replicate 2
Data processing use moabs to process bisulfite treated data
Genome_build: mm9
Supplementary_files_format_and_content: text; chromosome, start, end, methylation ratio, total reads, methylated reads, strand, next nucletide of cytosine, plus_strand, total reads from plus strand, methylated reads from plus strand, minus strand, total reads from minus strand, methylated reads from minus strand
 
Submission date Aug 10, 2013
Last update date May 15, 2019
Contact name Margaret Goodell
Organization name Baylor College of Medicine
Street address One Baylor Plaza, N1030
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL13112
Series (2)
GSE49191 Large conserved domains of low DNA methylation maintained by 5-hydroxymethycytosine and Dnmt3a
GSE49714 Large conserved domains of low DNA methylation maintained by 5-hydroxymethycytosine and Dnmt3a [Bisulfite-Seq]
Relations
BioSample SAMN02315120
SRA SRX333393

Supplementary file Size Download File type/resource
GSM1206263_m12_b4l1.bsmapv260R.bam.G.bed.gz 216.4 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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