|
Status |
Public on Jan 09, 2015 |
Title |
Stretch C |
Sample type |
SRA |
|
|
Source name |
Stretched AVIC
|
Organism |
Homo sapiens |
Characteristics |
cell type: aortic valve interstitial cells condition: Stretch
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using RNAeasy columns using the protocol that includes small RNAs The Illumina TruSeq Small RNA protocol was used. 1 microgram of total RNA was used. After adapter ligation, the small RNAs were gel purified according the manufactuers protocol.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Bowtie2 was used to map the sequence tags to the GRCh37.p10 human genome build edgeR algorithm in the Bioconducter suite was used to obtain expression levels and statistical significance Genome_build: GRCh37.p10
|
|
|
Submission date |
Aug 05, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Vishal Nigam |
E-mail(s) |
vishal.nigam@seattlechildrens.org
|
Organization name |
Seattle Childrens Research Institute
|
Department |
Pediatrics
|
Street address |
1900 9th Ave
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98101 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE49558 |
Stretch responsive miRNAs in human aortic valve interstitial cells |
|
Relations |
BioSample |
SAMN02303006 |
SRA |
SRX331697 |