|
| Status |
Public on Jan 09, 2015 |
| Title |
Static control A |
| Sample type |
SRA |
| |
|
| Source name |
Static AVIC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: aortic valve interstitial cells
condition: Static
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNAeasy columns using the protocol that includes small RNAs
The Illumina TruSeq Small RNA protocol was used. 1 microgram of total RNA was used. After adapter ligation, the small RNAs were gel purified according the manufactuers protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Bowtie2 was used to map the sequence tags to the GRCh37.p10 human genome build
edgeR algorithm in the Bioconducter suite was used to obtain expression levels and statistical significance
Genome_build: GRCh37.p10
|
| |
|
| Submission date |
Aug 05, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Vishal Nigam |
| E-mail(s) |
vishal.nigam@seattlechildrens.org
|
| Organization name |
Seattle Childrens Research Institute
|
| Department |
Pediatrics
|
| Street address |
1900 9th Ave
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98101 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE49558 |
Stretch responsive miRNAs in human aortic valve interstitial cells |
|
| Relations |
| BioSample |
SAMN02302998 |
| SRA |
SRX331689 |
