|
| Status |
Public on Aug 13, 2013 |
| Title |
Primary glioblastoma sample 5 |
| Sample type |
RNA |
| |
|
| Source name |
primary glioblastoma
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age (y): 58
tumor grade: IV
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small pieces of snap-frozen samples were homogenized in Trizol following manufacturer's instructions. Samples were then treated with DNase and further purified on Rneasy columns. RNA integrity was verified by Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
NuGEN Applause Amp Plus ST System and Encore Biotin Module
|
| |
|
| Hybridization protocol |
GeneChip Expression Analysis Technical Manual 702232 Rev 3
|
| Scan protocol |
GCS3000
|
| Description |
frozen surgical resection
|
| Data processing |
AGCC 3.2.2
probe group file: HuGene-1_0-st-v1.r4.pgf
meta-probeset file: HuGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Jul 31, 2013 |
| Last update date |
Aug 13, 2013 |
| Contact name |
RAMAN PAUL NAGARAJAN |
| Organization name |
UCSF
|
| Department |
NEUROSURGERY
|
| Lab |
COSTELLO
|
| Street address |
1450 3RD ST, ROOM HD200
|
| City |
SAN FRANCISCO |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL15648 |
| Series (2) |
| GSE49412 |
Expression data from five primary human glioblastomas (frozen surgical resection) and one non-neoplastic adult brain (frozen autopsy tissue) |
| GSE49810 |
Expression and copy number data from five primary human glioblastomas |
|
