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Sample GSM1197402 Query DataSets for GSM1197402
Status Public on Feb 04, 2014
Title SMP
Sample type RNA
Source name MDA-MB-468 shRNA scramble control cells
Organism Homo sapiens
Characteristics cell line: MDA-MB-468
cell type: breast cancer
Growth protocol Stable transfectants were maintained in DMEM supplemented with 10% FBS and 0.25ug/mL puromycin.
Extracted molecule total RNA
Extraction protocol RNA extracted with TRIzol and Purelink RNA kit
Label CY3
Label protocol 400ng of the RNA samples spiked with RNA spike in controls were converted to cDNA and in vitro transcribed for amplification and incorporation of labelled CTP (cRNA).
Hybridization protocol Purified cRNA samples were fragmented and hybridized to an Agilent SurePrint G3 8x60K Gene Expression microarray v2 slide
Scan protocol scanned at 5uM resolution with an Agilent G2565CA High Resolution Scanner
Description puromycin selected polyclonal cells
Data processing Data was processed with Agilent’s Feature Extraction software version using the protocol GE1_1100_Jul11 and 8x60K grid file 039494_D_F_20120628. Samples were normalized using the percentile shift algorithm with no baseline transformation.
Submission date Jul 29, 2013
Last update date Feb 04, 2014
Contact name Paola Marcato
Phone 9024944239
Organization name Dalhousie University
Department Pathology
Street address 11F11, 5850 College Street
City Halifax
State/province Nova Scotia
ZIP/Postal code B3H 4R2
Country Canada
Platform ID GPL17077
Series (1)
GSE49322 ALDH1A3-mediated gene expression in MDA-MB-231 cells and MDA-MB-468 cells

Supplementary file Size Download File type/resource
GSM1197402_US84903607_253949411345_S01_GE1_1100_Jul11_1_3.pdf.gz 115.2 Kb (ftp)(http) PDF
GSM1197402_US84903607_253949411345_S01_GE1_1100_Jul11_1_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data are available on Series record

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