|Public on Feb 04, 2014
|MDA-MB-468 shRNA scramble control cells
|cell line: MDA-MB-468
cell type: breast cancer
|Stable transfectants were maintained in DMEM supplemented with 10% FBS and 0.25ug/mL puromycin.
|RNA extracted with TRIzol and Purelink RNA kit
|400ng of the RNA samples spiked with RNA spike in controls were converted to cDNA and in vitro transcribed for amplification and incorporation of labelled CTP (cRNA).
|Purified cRNA samples were fragmented and hybridized to an Agilent SurePrint G3 8x60K Gene Expression microarray v2 slide
|scanned at 5uM resolution with an Agilent G2565CA High Resolution Scanner
|puromycin selected polyclonal cells
|Data was processed with Agilent’s Feature Extraction software version 126.96.36.199 using the protocol GE1_1100_Jul11 and 8x60K grid file 039494_D_F_20120628. Samples were normalized using the percentile shift algorithm with no baseline transformation.
|Jul 29, 2013
|Last update date
|Feb 04, 2014
|11F11, 5850 College Street
|ALDH1A3-mediated gene expression in MDA-MB-231 cells and MDA-MB-468 cells