|
Status |
Public on Feb 04, 2014 |
Title |
1A3 |
Sample type |
RNA |
|
|
Source name |
MDA-MB-231 ALDH1A3 overexpression cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: MDA-MB-231 cell type: breast cancer
|
Growth protocol |
Stable transfectants were maintained in DMEM supplemented with 10% FBS and 0.25ug/mL puromycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extracted with TRIzol and Purelink RNA kit
|
Label |
CY3
|
Label protocol |
400ng of the RNA samples spiked with RNA spike in controls were converted to cDNA and in vitro transcribed for amplification and incorporation of labelled CTP (cRNA).
|
|
|
Hybridization protocol |
Purified cRNA samples were fragmented and hybridized to an Agilent SurePrint G3 8x60K Gene Expression microarray v2 slide
|
Scan protocol |
scanned at 5uM resolution with an Agilent G2565CA High Resolution Scanner
|
Description |
puromycin selected polyclonal cells
|
Data processing |
Data was processed with Agilent’s Feature Extraction software version 11.0.1.1 using the protocol GE1_1100_Jul11 and 8x60K grid file 039494_D_F_20120628. Samples were normalized using the percentile shift algorithm with no baseline transformation.
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|
|
Submission date |
Jul 29, 2013 |
Last update date |
Feb 04, 2014 |
Contact name |
Paola Marcato |
E-mail(s) |
paola.marcato@dal.ca
|
Phone |
9024944239
|
Organization name |
Dalhousie University
|
Department |
Pathology
|
Street address |
11F11, 5850 College Street
|
City |
Halifax |
State/province |
Nova Scotia |
ZIP/Postal code |
B3H 4R2 |
Country |
Canada |
|
|
Platform ID |
GPL17077 |
Series (1) |
GSE49322 |
ALDH1A3-mediated gene expression in MDA-MB-231 cells and MDA-MB-468 cells |
|