|
| Status |
Public on May 16, 2014 |
| Title |
J1_Ino80_siRNA_2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse J1 ESC, Ino80 siRNA, replicate 2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Mouse embryonic stem cell line
cell line: Mouse J1 ESC
agent: Ino80_siRNA
time: Day_4
group: Ino80_siRNA
control group: NT_siRNA
|
| Treatment protocol |
Cell were transfected with siRNAs against NT, Ino80, Ino80b, Ino80c or Ino80e, and harvested 96 hours after transfection. Cells were infected with shRNA-NT or shRNA-Ino80, and harvested 2, 4 or 6 days after infection.
|
| Growth protocol |
J1 cell were maintianed in ESGRO medium. Cell were cultured in M15 medium when transfection or infection was performed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were prepared using Therno RNA kit according to the manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Quick Amp labeling kit
|
| |
|
| Hybridization protocol |
Based on standard Agilent one-color protocol
|
| Scan protocol |
Images were scanned with an Agilent DNA Microarray Scanner (G2505B), and analyzed with Agilent Feature Extraction Software version 9.5.3.1 using default parameters (Protocol: GE1-v5_95_Feb07 and Grid: 014868_D_F_20060807.)
|
| Description |
Gene expression 96 hours after transfection
|
| Data processing |
Agilent signal intensities were loaded by Agi4x44PreProcess 1.20.0, the G signal was normalized to median log2 fold change of zero.
|
| |
|
| Submission date |
Jul 26, 2013 |
| Last update date |
May 16, 2014 |
| Contact name |
Li Wang |
| Organization name |
NIH
|
| Street address |
111 T.W. Alexander Dr
|
| City |
Durham |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (2) |
| GSE49251 |
INO80 complex in the core regulatory network governing ESC self-renewal [728_subunits] |
| GSE49252 |
INO80 complex in the core regulatory network governing ESC self-renewal |
|
