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Sample GSM1194965 Query DataSets for GSM1194965
Status Public on Sep 01, 2013
Title Cumulus_clone_fusion_blastocyst_rep4
Sample type RNA
Source name cumulus clone, cell fusion, blastocyst
Organism Mus musculus
Characteristics gender: female
donor cell: cumulus cell
tissue: blastocyst
embryonic day: E4.0
Treatment protocol Embryos that reached the blastocyst (E4.0) stage were used to extract total RNA.
Growth protocol In vitro fertilized (IVF) or cloned embryos were cultured in potassium modified simplex optimization medium (KSOM) at 37.5 ºC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with TRIzol (Life Technologies) from epiblasts or extraembryonic regions derived from single embryos and subjected to linear amplification using TargetAmp Two-Round Aminoallyl-aRNA Amplification Kits (Epicentre Biotechnologies).
Label Cy3
Label protocol 5.0 ug of amplified RNA was labelled with Cianine-3 (Cy3) dye (GE Healthcare) for 90 minutes at RT. Labelled RNAs were purified with RNeasy MinElute kit (Qiagen) and checked with the NanoDrop ND-1000 Spectrophotometer
Hybridization protocol 1.65ug Cy3-labelled RNAs were fragmented at 60°C for 30 minutes and hybridized at 65°C for 17 hours according to manufacture's instructions.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting (Scan Area 61x21.6 mm, Scan resolution 5 um, Dye channel is set to Green and Green PMT is set to 100%).
Description cumulus clone, cell fusion, blastocyst, replicate4
Data processing The scanned images of microarray slides were processed using Feature Extraction software 10.5.1 (Agilent Technologies). All raw data were loaded into Gene Spring GX 12.5 (Agilent Technologies) and transformed by default setting.
Submission date Jul 24, 2013
Last update date Sep 01, 2013
Contact name Kimiko Inoue
Organization name BRC, RIKEN
Department Bioresource Engineering Division
Street address 3-1-1 Koyadai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-0074
Country Japan
Platform ID GPL7202
Series (2)
GSE49172 Comparative gene expression analyses using E4.0 cloned blastocysts
GSE49173 Donor cell type-specific gene expression in embryonic but not extraembryonic tissues of postimplantation embryos cloned from somatic cells

Data table header descriptions
VALUE Normalized data expressed in log2 scale.

Data table
A_52_P616356 0.501174
A_52_P580582 -0.3057332
A_52_P403405 0.3571496
A_52_P819156 3.330841
A_51_P331831 0.38694572
A_51_P430630 0
A_52_P502357 0.4520769
A_52_P299964 -0.004326344
A_51_P356389 -0.41323948
A_52_P684402 1.0817337
A_51_P414208 0.21591139
A_51_P280918 0
A_52_P613688 0
A_52_P258194 -0.56482553
A_52_P229271 -1.5818686
A_52_P214630 0.45410442
A_52_P579519 -0.18829823
A_52_P979997 0.48404312
A_52_P453864 0.4879532
A_52_P655842 0.07458782

Total number of rows: 41265

Table truncated, full table size 942 Kbytes.

Supplementary file Size Download File type/resource
GSM1194965_CC4_fus_blastocyst.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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