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Sample GSM1194053 Query DataSets for GSM1194053
Status Public on Mar 01, 2014
Title JQ1 treatment, biological rep1
Sample type RNA
 
Source name JQ1-treated MCF7 cells, 0.2 uM, 24 hours
Organism Homo sapiens
Characteristics cell line: MCF7
treatment: JQ1 (0.2 uM) for 24 hours
phenotype: tamoxifen-resistant
Treatment protocol tamoxifen resistant MCF7 cells were maintained in phenol red-free medium containing 10% charcoal-dextran-stripped FCS and 100 nM of 4-HT. JQ1 or DMSO was added and incubated for 24 hours before harvest for RNA purification.
Growth protocol tamoxifen-resistant MCF7 cells were cultured in phenol red-free medium containing 10% charcoal-dextran-stripped FCS and 100 nM of 4-HT.
Extracted molecule total RNA
Extraction protocol Qiagen Rneasy kit was used for total RNA extraction according to the manufacturer's instructions.
Label biotinylated
Label protocol cDNA were prepared according to the standard Affymetrix protocol for whole transcriptome analysis from 100ng total RNA.
 
Hybridization protocol Following end-labeling with biotin, 2 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using an Affymetrix 3000 scanner with 7G upgrade
Description gene expression data from JQ1-treated MCF7 cells, 0.2 uM, 24 hours
Data processing The data were analyzed with Affymetrix Expression Console using Affymetrix default analysis settings and RMA as normalization method on core dataset.
 
Submission date Jul 23, 2013
Last update date Mar 01, 2014
Contact name Qin Feng
E-mail(s) qfeng4@central.uh.edu
Phone 8328428824
Organization name University of Houston
Department Biology and Biochemistry
Street address 3517 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77204
Country USA
 
Platform ID GPL10739
Series (1)
GSE49124 Expression data from JQ1 (0.2 uM) treated tamoxifen-resistant MCF7 cells

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
7896737 6.182958
7896739 3.693478
7896741 4.196836
7896743 7.364841
7896745 5.173517
7896747 8.748061
7896749 8.144465
7896751 4.066025
7896753 10.42432
7896755 6.275628
7896757 5.846294
7896758 5.495337
7896760 6.221627
7896762 11.12085
7896763 7.578317
7896764 8.094992
7896765 3.627027
7896766 6.981812
7896767 5.632503
7896768 10.82737

Total number of rows: 257430

Table truncated, full table size 4244 Kbytes.




Supplementary file Size Download File type/resource
GSM1194053_A0062908.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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