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Sample GSM1194052 Query DataSets for GSM1194052
Status Public on Mar 01, 2014
Title DMSO treatment, biological rep3
Sample type RNA
Source name DMSO-treated MCF7 cells, 24 hours
Organism Homo sapiens
Characteristics cell line: MCF7
treatment: DMSO-treated for 24 hours
phenotype: tamoxifen-resistant
Treatment protocol tamoxifen resistant MCF7 cells were maintained in phenol red-free medium containing 10% charcoal-dextran-stripped FCS and 100 nM of 4-HT. JQ1 or DMSO was added and incubated for 24 hours before harvest for RNA purification.
Growth protocol tamoxifen-resistant MCF7 cells were cultured in phenol red-free medium containing 10% charcoal-dextran-stripped FCS and 100 nM of 4-HT.
Extracted molecule total RNA
Extraction protocol Qiagen Rneasy kit was used for total RNA extraction according to the manufacturer's instructions.
Label biotinylated
Label protocol cDNA were prepared according to the standard Affymetrix protocol for whole transcriptome analysis from 100ng total RNA.
Hybridization protocol Following end-labeling with biotin, 2 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using an Affymetrix 3000 scanner with 7G upgrade
Description gene expression data from DMSO-treated MCF7 cells, 24 hours
Data processing The data were analyzed with Affymetrix Expression Console using Affymetrix default analysis settings and RMA as normalization method on core dataset.
Submission date Jul 23, 2013
Last update date Mar 01, 2014
Contact name Qin Feng
Phone 8328428824
Organization name University of Houston
Department Biology and Biochemistry
Street address 3517 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77204
Country USA
Platform ID GPL10739
Series (1)
GSE49124 Expression data from JQ1 (0.2 uM) treated tamoxifen-resistant MCF7 cells

Data table header descriptions
VALUE RMA normalized

Data table
7896737 5.908748
7896739 3.471546
7896741 3.845055
7896743 6.844539
7896745 4.771374
7896747 8.340775
7896749 7.247783
7896751 3.551351
7896753 9.47293
7896755 6.184402
7896757 5.840499
7896758 5.462113
7896760 6.739392
7896762 11.37469
7896763 7.059583
7896764 8.098706
7896765 3.525788
7896766 6.860989
7896767 6.578945
7896768 11.04292

Total number of rows: 257430

Table truncated, full table size 4248 Kbytes.

Supplementary file Size Download File type/resource
GSM1194052_A0062907.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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