|
| Status |
Public on Jul 17, 2024 |
| Title |
NHU-hTERT cells with Stable expression of ARL11 after 48 hours B |
| Sample type |
RNA |
| |
|
| Source name |
human normal urothelial limmortalized cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: NHU-hTERT
transfection: ARL11-lenti viral vector
|
| Treatment protocol |
NHU-hTERT cells with empty vector and stable expression of ARL11 were starved for 2 days before the harvest.
|
| Growth protocol |
NHU-hTERT cells stably transfected with empty and ARL11-lenti viral vector were grown in Keratinocyte-SFM medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Quantile normalization in the Linear Model for Microarray Data (LIMMA)
|
| |
|
| Submission date |
Jul 18, 2013 |
| Last update date |
Jul 17, 2024 |
| Contact name |
Bogdan Czerniak |
| E-mail(s) |
bczernia@mdanderson.org
|
| Organization name |
UT MD Anderson Cancer Center
|
| Department |
Pathology
|
| Street address |
1515 Holcombe
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE49022 |
ARL11 Stable Expression in NHU-hTERT cells |
|
