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Sample GSM1191059 Query DataSets for GSM1191059
Status Public on Jul 19, 2013
Title Human adult skin fibroblast 1
Sample type RNA
 
Source name Skin
Organism Homo sapiens
Characteristics tissue: Skin
gender: female
Extracted molecule total RNA
Extraction protocol RNA was extracted using an RNeasy Plus Micro Kit with a genomic DNA Eliminator (QIAGEN) according to the manufacturer's instructions. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
 
Hybridization protocol 300 ng of Cy3-labelled cRNA was fragmented 1x Agilent fragmentation buffer and hybridized to Agilent SurePrint G3 Human GE Microarray ( x 60K) for 17 hours at 65°C in a rotating Agilent hybridization oven.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
Data processing The scanned images were analyzed with Feature Extraction Software (v.10.7.3.1) (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
 
Submission date Jul 17, 2013
Last update date Jul 19, 2013
Contact name Rimpei Morita
E-mail(s) rimpeim@a7.keio.jp
Organization name Keio University School of Medicine
Street address 35 Shinanomachi, Shinjyuku-ku
City Tokyo
ZIP/Postal code 160-8582
Country Japan
 
Platform ID GPL17077
Series (1)
GSE48980 Profiling gene expression of ETV2-induced vascular endothelial cells (ETVECs)

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 122525.73
DarkCorner 29.440216
A_23_P117082 9078.704
A_33_P3246448 131.29831
A_33_P3318220 4.590665
A_33_P3236322 4.613628
A_33_P3319925 4.6342244
A_21_P0000509 63169.58
A_21_P0000744 1779.5756
A_24_P215804 673.31323
A_23_P110167 1009.69635
A_33_P3211513 109.35221
A_23_P103349 4.3856773
A_32_P61480 4.7245545
A_33_P3788124 4.7302775
A_33_P3414202 77.15464
A_33_P3316686 193.29683
A_33_P3300975 3702.1042
A_33_P3263061 6084.379
A_33_P3261373 4.73539

Total number of rows: 50739

Table truncated, full table size 1142 Kbytes.




Supplementary file Size Download File type/resource
GSM1191059_HAF1.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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