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Sample GSM1191058 Query DataSets for GSM1191058
Status Public on Jul 19, 2013
Title ETV2-indeuced vascular endothelial cells 3
Sample type RNA
Source name Culture cells
Organism Homo sapiens
Characteristics tissue: Culture cells
gender: female
Extracted molecule total RNA
Extraction protocol RNA was extracted using an RNeasy Plus Micro Kit with a genomic DNA Eliminator (QIAGEN) according to the manufacturer's instructions. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
Hybridization protocol 300 ng of Cy3-labelled cRNA was fragmented 1x Agilent fragmentation buffer and hybridized to Agilent SurePrint G3 Human GE Microarray ( x 60K) for 17 hours at 65°C in a rotating Agilent hybridization oven.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
Data processing The scanned images were analyzed with Feature Extraction Software (v. (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
Submission date Jul 17, 2013
Last update date Jul 19, 2013
Contact name Rimpei Morita
Organization name Keio University School of Medicine
Street address 35 Shinanomachi, Shinjyuku-ku
City Tokyo
ZIP/Postal code 160-8582
Country Japan
Platform ID GPL17077
Series (1)
GSE48980 Profiling gene expression of ETV2-induced vascular endothelial cells (ETVECs)

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 117316.305
DarkCorner 6.8408885
A_23_P117082 18238.715
A_33_P3246448 828.2371
A_33_P3318220 6.688707
A_33_P3236322 6.722657
A_33_P3319925 6.7526636
A_21_P0000509 5407.8765
A_21_P0000744 840.6323
A_24_P215804 593.4184
A_23_P110167 4945.093
A_33_P3211513 99.930176
A_23_P103349 103.74222
A_32_P61480 6.8825903
A_33_P3788124 6.8907204
A_33_P3414202 63.325806
A_33_P3316686 203.45773
A_33_P3300975 743.36975
A_33_P3263061 7153.969
A_33_P3261373 6.899889

Total number of rows: 50739

Table truncated, full table size 1143 Kbytes.

Supplementary file Size Download File type/resource
GSM1191058_ETVEC3.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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