|
| Status |
Public on Jul 16, 2016 |
| Title |
Spink3-/- rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Spink3-/-_pancreas_p0.5
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6N
genotype/variation: Spink3-/-
developmental age: p0.5
tissue: whole pancreas
|
| Treatment protocol |
The pancreas was harvested at p0.5, and frozen by liquid nitrogen immediately.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the RNeasy Mini Kit (Cat. no. 74106, Qiagen, Tokyo, Japan) following the manufacturer's recommendations. DNA was digested byRQ1 RNase-Free DNase (Promega, Madison, WI). RNA was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Yokohama, Japan).
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution.
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 028005_D_F_20110722) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Jul 17, 2013 |
| Last update date |
Jul 16, 2016 |
| Contact name |
Masaki Ohmuraya |
| E-mail(s) |
ohmuraya@kumamoto-u.ac.jp
|
| Phone |
+81-96-373-6818
|
| Organization name |
Kumamoto University
|
| Department |
Institute of Resource Development and
|
| Lab |
Division of Transgenic Technology
|
| Street address |
2-2-1
|
| City |
Honjo, Chuo-ku |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE48946 |
Gene expression of non-apoptotic pancreatic acinar cell death with autophagy |
|
