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| Status |
Public on Apr 03, 2008 |
| Title |
AND T cells-short dox, PBS-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
lymph node AND T cells, short dox, PBS
|
| Organism |
Mus musculus |
| Characteristics |
Genetic background: B10.BR,
Transgene donor cells: AND T cell receptor genes,
Transgenes recipients: Ii-rTA+, TIM+,
Age: 6-8 weeks,
Tissue: subcutaneous lymph nodes,
Isolation: FACS on CD4+CD90.1+ cells
|
| Treatment protocol |
dox from d-1 to d0, PBS ip on d-1, AND cells iv on d0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
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| |
|
| Description |
AND CD4+ T cells, transient antigen expression, no DC activation. jos3059
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jul 06, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5245 |
Profiling of CD4+ T cells responding to transient or persistent antigen presented by dendritic cells in vivo |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Data table header descriptions |
| ID_REF |
|
| VALUE |
signal |
| Data table |
| ID_REF |
VALUE |
| 1415670_at |
67.666518 |
| 1415671_at |
221.210144 |
| 1415672_at |
1374.874698 |
| 1415673_at |
183.324367 |
| 1415674_a_at |
161.835144 |
| 1415675_at |
206.710144 |
| 1415676_a_at |
512.199723 |
| 1415677_at |
97.417995 |
| 1415678_at |
438.97062 |
| 1415679_at |
1015.295575 |
| 1415680_at |
153.085144 |
| 1415681_at |
129.055069 |
| 1415682_at |
78.918432 |
| 1415683_at |
653.965207 |
| 1415684_at |
69.422293 |
| 1415685_at |
103.58837 |
| 1415686_at |
543.149422 |
| 1415687_a_at |
750.214171 |
| 1415688_at |
786.487968 |
| 1415689_s_at |
179.709094 |
Total number of rows: 45101
Table truncated, full table size 934 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM118674.CEL.gz |
5.6 Mb |
(ftp)(http) |
CEL |
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