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| Status |
Public on Apr 03, 2008 |
| Title |
AND T cells-short dox, PBS-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
lymph node AND T cells, short dox, PBS
|
| Organism |
Mus musculus |
| Characteristics |
Genetic background: B10.BR,
Transgene donor cells: AND T cell receptor genes,
Transgenes recipients: Ii-rTA+, TIM+,
Age: 6-8 weeks,
Tissue: subcutaneous lymph nodes,
Isolation: FACS on CD4+CD90.1+ cells
|
| Treatment protocol |
dox from d-1 to d0, PBS ip on d-1, AND cells iv on d0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
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| |
|
| Description |
AND CD4+ T cells, transient antigen expression, no DC activation. jos3046
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jul 06, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5245 |
Profiling of CD4+ T cells responding to transient or persistent antigen presented by dendritic cells in vivo |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Data table header descriptions |
| ID_REF |
|
| VALUE |
signal |
| Data table |
| ID_REF |
VALUE |
| 1415670_at |
96.438507 |
| 1415671_at |
189.707149 |
| 1415672_at |
1322.163927 |
| 1415673_at |
172.904378 |
| 1415674_a_at |
143.960144 |
| 1415675_at |
159.37081 |
| 1415676_a_at |
377.000284 |
| 1415677_at |
86.145315 |
| 1415678_at |
333.859272 |
| 1415679_at |
828.004472 |
| 1415680_at |
130.082226 |
| 1415681_at |
74.760425 |
| 1415682_at |
96.440057 |
| 1415683_at |
603.335144 |
| 1415684_at |
77.354582 |
| 1415685_at |
76.030825 |
| 1415686_at |
385.815519 |
| 1415687_a_at |
376.455812 |
| 1415688_at |
613.105333 |
| 1415689_s_at |
112.397644 |
Total number of rows: 45101
Table truncated, full table size 934 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM118671.CEL.gz |
5.8 Mb |
(ftp)(http) |
CEL |
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