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Sample GSM1186209 Query DataSets for GSM1186209
Status Public on Nov 06, 2014
Title adult progenitor of oligodendrocytes from control brains_rep2
Sample type RNA
 
Source name adult progenitor of oligodendrocytes from control brains
Organism Mus musculus
Characteristics strain: CR57BL/6
cell type: Primary FAC-sorted adult OPCs
treatment: Control
Extracted molecule total RNA
Extraction protocol Total RNAs were obtained from FAC-sorted neonatal or 2-month-old progenitors of oligodendrocytes or oligodendrocytes, isolated from mice treated or not with cuprizone for 5 weeks. We used Nucleospin RNA XS kit (Macherey Nagel, France) in accordance with the manufacturer’s protocol. The quality and quantity of each RNA preparation was assessed on the Agilent 2100 Bioanalyzer with RNA 6000 PicoChips (Agilent Technologies).
Label Cy3
Label protocol Ten nanograms of each RNA were amplified by IVT and labeled with Cy3 using the Low Imput Quick Amp labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
 
Hybridization protocol Five hundred nanogams of each Cy3-cRNA were hybridized overnight at 65°C on Whole Mouse Genome Microarray 4x44K v2 (Agilent Technologies) according to the manufacturer’s instructions.
Scan protocol Microarrays data were acquired on an InnoScan 900 (Innopsys, France) with a resolution of 5 µm
Data processing We scanned the chips using Nimbile Gem MS 200 (Roche).
Raw data were obtained with Mapix 5.0.0 software (Innopsys, France). For each sample, raw data consisted of the Median Feature Intensity – Median Background Intensity (F-B) at wavelength 532nm.
We then used R to normalize our data.
 
Submission date Jul 15, 2013
Last update date Nov 06, 2014
Contact name Sarah Moyon
E-mail(s) smoyon@gc.cuny.edu
Organization name CUNY ASRC
Department Neuroscience
Lab Casaccia
Street address 85 St Nicholas Terrace
City New York
State/province NY
ZIP/Postal code 10031
Country USA
 
Platform ID GPL11202
Series (1)
GSE48872 After demyelination in the adult CNS, oligodendrocyte precursor cells revert to an immature mRNA profile and express immune cues favoring their migration

Data table header descriptions
ID_REF
VALUE Log2 quantile-normalized signal intensity

Data table
ID_REF VALUE
(-)3xSLv1
(+)E1A_r60_1
(+)E1A_r60_3
(+)E1A_r60_a104
(+)E1A_r60_a107 7.471916707
(+)E1A_r60_a135
(+)E1A_r60_a20
(+)E1A_r60_a22 6.620807391
(+)E1A_r60_a97
(+)E1A_r60_n11
(+)E1A_r60_n9 7.109130755
A_51_P100034 11.99228459
A_51_P100174 6.796747323
A_51_P100208 6.493007518
A_51_P100289 10.07469517
A_51_P100298
A_51_P100309
A_51_P100327
A_51_P100347 7.706437588
A_51_P100519

Total number of rows: 39485

Table truncated, full table size 865 Kbytes.




Supplementary file Size Download File type/resource
GSM1186209_raw_data_NonactivatedaOPCs_2.txt.gz 6.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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