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Sample GSM1183145 Query DataSets for GSM1183145
Status Public on Oct 23, 2014
Title Combination P18-2
Sample type RNA
Source name prostate tumor_OMN54+Docetaxel
Organism Homo sapiens
Characteristics host strain: NOD-SCID mice
host strain gender: male
cell line source: LTL-313H
tissue type: patient-derived prostate cancer tissue xenograft
treated with: OMN54 + Docetaxel in combination
Treatment protocol After about 6 weeks, when levels of ~12 ng PSA/ml plasma had been reached, i.e. equivalent to tumor volumes of 30-50 mm^3, the mice were randomly distributed into 6 groups and treated with docetaxel (ip; Q7d/3) and Aneustat (orally; Q1dX5/3) along the following schedule: (a) vehicle control, (b) docetaxel (5 mg/kg), (c) Aneustat (1652 mg/kg), (d) docetaxel (5 mg/kg) + Aneustat (413 mg/kg), (e) docetaxel (5 mg/kg) + Aneustat (826 mg/kg), and (f) docetaxel (5 mg/kg) + Aneustat (1652 mg/kg). Expression microarray data were obtained from LTL-313H xenografts treated under four different conditions: docetaxel (5 mg/kg), Aneustat (1652 mg/kg), docetaxel (5 mg/kg) + Aneustat (1652 mg/kg), and untreated controls. Aneustat and docetaxel were both dissolved in DMSO and added as single drugs or in various combinations. DMSO was used as the vehicle control.
Growth protocol The LTL-313H transplantable, PTEN-deficient, metastatic and PSA-secreting, patient-derived prostate cancer tissue line (generation 13) was maintained as grafts under renal capsules of male NOD-SCID mice supplemented with testosterone as described in manuscript. For experiments, tumors were harvested 10 weeks after grafting and pieces of tumor tissue (2.5x2.5x1.25 mm3) were grafted under the renal capsules of 36 testosterone-supplemented male mice (6 groups; 6 mice/group; 4 grafts/mouse). The grafts had a 100% engraftment rate with an average tumor volume doubling time of 13-15 days. Increases in the plasma PSA levels of the mice were used as a measure of tumor growth.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from xenograft tissues using a mirVana™ miRNA Isolation Kit (Life Technologies, Burlington, ON, Canada) according to the manufacturer's instructions.
Label Cy3
Label protocol 100ng of total RNA was used as input for generation of Cyanine-3-labeled cRNA following Agilent’s One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling v6.0.
Hybridization protocol 600ng labeled cRNA was hybridized on Agilent SurePrint G3 Human GE 8x60K Microarray (Design ID 028004)
Scan protocol Slides were scanned with the Agilent DNA Microarray Scanner at a 3um scan resolution using the one colour and 20 bit tiff file settings
Data processing Image files were processed with Agilent Feature Extraction to produce spatially detrended and background subtracted gProcessedSignal intensities. Processed green signal was quantile normalized with Agilent GeneSpring 12.0.
Submission date Jul 10, 2013
Last update date Apr 23, 2018
Contact name Shawn Anderson
Organization name Vancouver Prostate Centre
Lab Laboratory for Advanced Genome Analysis
Street address 2660 Oak Street
City Vancouver
State/province BC
ZIP/Postal code V6H3Z6
Country Canada
Platform ID GPL17077
Series (1)
GSE48667 Enhancement by Aneustat (OMN54) of docetaxel anticancer activity in a patient-derived prostate cancer tissue xenograft model
Reanalyzed by GSE113533

Data table header descriptions
VALUE log2 quantile normalized

Data table
A_23_P117082 12.2002325
A_33_P3246448 5.5070376
A_33_P3318220 3.2954116
A_33_P3236322 4.8955936
A_33_P3319925 6.311507
A_21_P0000509 15.3699255
A_21_P0000744 11.032162
A_24_P215804 7.5094543
A_23_P110167 12.984158
A_33_P3211513 9.285167
A_23_P103349 2.2128553
A_32_P61480 4.0222716
A_33_P3788124 2.991428
A_33_P3414202 6.425969
A_33_P3316686 7.2834845
A_33_P3300975 10.502892
A_33_P3263061 11.556558
A_33_P3261373 1.5048714
A_24_P278460 9.1498575
A_21_P0013109 1.5026561

Total number of rows: 50737

Table truncated, full table size 1145 Kbytes.

Supplementary file Size Download File type/resource
GSM1183145_US09503746_253949410063_S01_GE1_1100_Jul11_1_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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