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Sample GSM118226 Query DataSets for GSM118226
Status Public on Jul 02, 2007
Title ftsY disruption, replicate 1
Sample type RNA
 
Channel 1
Source name ftsY disruption
Organism Bacillus subtilis
Characteristics Strain: RB25
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name collective reference sample
Organism Bacillus subtilis
Characteristics Mixture of wild-type RNA
Extracted molecule total RNA
Label Cy5
 
 
Description See Britton RA et al. J Bacteriol. 2006 for protocol details.
Data processing Cy3 and Cy5 signals for each spot were normalized to the total Cy3 and Cy5 signals of the array. The total signal for each channel was calculated by summing the values of the total signals for each spot, which was calculated by multiplying the mean signal intensity (per pixel) by the number of pixels. Ratios were calculated only when the spot had a signal 1 standard deviation above background for 50% of pixels in each channel.
 
Submission date Jul 02, 2006
Last update date Jul 05, 2006
Contact name Alan D. Grossman
E-mail(s) clee2@mit.edu, adg@mit.edu
Phone 617-253-6702
Organization name MIT
Department Biology
Lab Grossman
Street address 31 Ames Street, 68-530
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL3950
Series (1)
GSE5210 Effects of the Structural Maintenance of Chromosomes (SMC) protein on gene expression in Bacillus subtilis

Data table header descriptions
ID_REF
VALUE Ratio of Ch1 signal to Ch2 signal normalized to total signal in each channel

Data table
ID_REF VALUE
WGD11-1 -0.71
WGD11-2 -0.10
WGD11-3 -0.04
WGD11-4 -0.40
WGD11-5 -0.34
WGD11-6 -0.48
WGD11-7 -0.23
WGD11-8 -0.50
WGD11-9 -0.03
WGD11-10 -0.21
WGD11-11 -0.05
WGD11-12 0.18
WGD11-13 -0.23
WGD11-14 -0.28
WGD11-15 -0.15
WGD11-16 -0.10
WGD11-17 -0.31
WGD11-18 -0.35
WGD11-19 -0.25
WGD11-20 -0.38

Total number of rows: 4120

Table truncated, full table size 66 Kbytes.




Supplementary file Size Download File type/resource
GSM118226.gpr.gz 311.1 Kb (ftp)(http) GPR

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