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Sample GSM118220 Query DataSets for GSM118220
Status Public on Jul 02, 2007
Title wild-type vs. smc deletion, replicate 2
Sample type RNA
 
Channel 1
Source name Wild-type
Organism Bacillus subtilis
Characteristics Strain: AG174
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name smc deletion
Organism Bacillus subtilis
Characteristics Strain: RB35
Extracted molecule total RNA
Label Cy5
 
 
Description See Britton RA et al. J Bacteriol. 2006 for protocol details.
Data processing Cy3 and Cy5 signals for each spot were normalized to the total Cy3 and Cy5 signals of the array. The total signal for each channel was calculated by summing the values of the total signals for each spot, which was calculated by multiplying the mean signal intensity (per pixel) by the number of pixels. Ratios were calculated only when the spot had a signal 1 standard deviation above background for 50% of pixels in each channel.
 
Submission date Jul 02, 2006
Last update date Jul 05, 2006
Contact name Alan D. Grossman
E-mail(s) clee2@mit.edu, adg@mit.edu
Phone 617-253-6702
Organization name MIT
Department Biology
Lab Grossman
Street address 31 Ames Street, 68-530
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL3949
Series (1)
GSE5210 Effects of the Structural Maintenance of Chromosomes (SMC) protein on gene expression in Bacillus subtilis

Data table header descriptions
ID_REF
VALUE Ratio of Ch1 signal to Ch2 signal normalized to total signal in each channel

Data table
ID_REF VALUE
WGD3-1 0.240753941
WGD3-2 0.686540866
WGD3-3 -0.179403559
WGD3-4 1.440229341
WGD3-5 0.336260533
WGD3-6 -0.268907147
WGD3-7 -0.175747007
WGD3-8 -0.385167679
WGD3-9 -0.484253239
WGD3-10 0.337083076
WGD3-11 -0.049377806
WGD3-12
WGD3-13 0.340676199
WGD3-14 0.106844098
WGD3-15
WGD3-16 0.498199798
WGD3-17 -0.241678059
WGD3-18 0.097947836
WGD3-19 0.223916751
WGD3-20 0.289810863

Total number of rows: 4099

Table truncated, full table size 80 Kbytes.




Supplementary file Size Download File type/resource
GSM118220.gpr.gz 296.3 Kb (ftp)(http) GPR

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