GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1180563 Query DataSets for GSM1180563
Status Public on Jul 04, 2013
Title Bone marrow 100 clone LOW
Sample type RNA
Source name MSC clone after 28 days of culture, CD146Low
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
surface cd146: low
donor: 100
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur 100 LOW
Gene expression data from human cultivated bone marrow-derived MSC during 28 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
VALUE GC-RMA normalized signal intensity

Data table
7892501 36.7528
7892502 34.9944
7892503 8.21132
7892504 325.862
7892505 9.1552
7892506 27.4301
7892507 9.09612
7892508 33.0891
7892509 2167.09
7892510 22.3843
7892511 7.52557
7892512 410.934
7892513 6.1049
7892514 1490.98
7892515 362.275
7892516 24.3562
7892517 18.4827
7892518 3.75219
7892519 32.8078
7892520 474.911

Total number of rows: 257430

Table truncated, full table size 4002 Kbytes.

Supplementary file Size Download File type/resource
GSM1180563_11.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap