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Sample GSM1180562 Query DataSets for GSM1180562
Status Public on Jul 04, 2013
Title Bone marrow 100 non-clonal
Sample type RNA
 
Source name MSC non-clonal after 21 days of culture
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
donor: 100
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
 
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur100 NC
Gene expression data from human cultivated bone marrow-derived MSC during 21 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
 
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
E-mail(s) nicolas.espagnolle@efs.sante.fr
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
 
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
ID_REF
VALUE GC-RMA normalized signal intensity

Data table
ID_REF VALUE
7892501 78.7296
7892502 35.1631
7892503 12.185
7892504 407.033
7892505 10.0011
7892506 27.5517
7892507 31.3373
7892508 13.8978
7892509 2977.43
7892510 18.6156
7892511 7.29944
7892512 207.913
7892513 6.85711
7892514 1596.14
7892515 426.838
7892516 29.4766
7892517 38.0156
7892518 2.97123
7892519 36.813
7892520 564.974

Total number of rows: 257430

Table truncated, full table size 4005 Kbytes.




Supplementary file Size Download File type/resource
GSM1180562_10.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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