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Sample GSM1180561 Query DataSets for GSM1180561
Status Public on Jul 04, 2013
Title Bone marrow 44 clone HIGH
Sample type RNA
Source name MSC clone after 28 days of culture, CD146High
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
surface cd146: high
donor: 44
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur44 HIGH
Gene expression data from human cultivated bone marrow-derived MSC during 28 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
VALUE GC-RMA normalized signal intensity

Data table
7892501 86.1704
7892502 67.7319
7892503 20.7419
7892504 508.497
7892505 13.7908
7892506 43.7188
7892507 15.7923
7892508 27.1049
7892509 2102.86
7892510 22.2784
7892511 9.73487
7892512 263.252
7892513 5.77283
7892514 1192.08
7892515 442.111
7892516 13.1724
7892517 48.4103
7892518 5.96993
7892519 40.7318
7892520 581.436

Total number of rows: 257430

Table truncated, full table size 4002 Kbytes.

Supplementary file Size Download File type/resource
GSM1180561_9.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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