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Sample GSM1180560 Query DataSets for GSM1180560
Status Public on Jul 04, 2013
Title Bone marrow 44 clone LOW
Sample type RNA
Source name MSC clone after 28 days of culture, CD146Low
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
surface cd146: low
donor: 44
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur 44 LOW
Gene expression data from human cultivated bone marrow-derived MSC during 28 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
VALUE GC-RMA normalized signal intensity

Data table
7892501 58.842
7892502 49.9347
7892503 10.6883
7892504 422.025
7892505 23.5547
7892506 40.1536
7892507 18.7826
7892508 8.83804
7892509 2480.87
7892510 21.5225
7892511 8.98029
7892512 296.182
7892513 11.0435
7892514 1328.35
7892515 411.164
7892516 7.68643
7892517 53.7974
7892518 4.20535
7892519 56.5617
7892520 675.299

Total number of rows: 257430

Table truncated, full table size 4001 Kbytes.

Supplementary file Size Download File type/resource
GSM1180560_8.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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