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Sample GSM1180559 Query DataSets for GSM1180559
Status Public on Jul 04, 2013
Title Bone marrow 44 non-clonal
Sample type RNA
Source name MSC non-clonal after 21 days of culture
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
donor: 44
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur44 NC
Gene expression data from human cultivated bone marrow-derived MSC during 21 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
VALUE GC-RMA normalized signal intensity

Data table
7892501 25.5232
7892502 37.4465
7892503 10.6446
7892504 254.307
7892505 6.86136
7892506 30.6244
7892507 8.63338
7892508 19.0533
7892509 2311.57
7892510 27.6103
7892511 9.5499
7892512 198.125
7892513 11.4137
7892514 1293.3
7892515 407.284
7892516 8.13402
7892517 27.1605
7892518 4.10383
7892519 35.158
7892520 578.956

Total number of rows: 257430

Table truncated, full table size 4002 Kbytes.

Supplementary file Size Download File type/resource
GSM1180559_7.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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