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Sample GSM1180558 Query DataSets for GSM1180558
Status Public on Jul 04, 2013
Title Bone marrow 122 clone HIGH
Sample type RNA
Source name MSC clone after 28 days of culture, CD146High
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
surface cd146: high
donor: 122
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur122 HIGH
Gene expression data from human cultivated bone marrow-derived MSC during 28 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
VALUE GC-RMA normalized signal intensity

Data table
7892501 54.1037
7892502 48.7615
7892503 9.62175
7892504 433.599
7892505 5.27191
7892506 38.7002
7892507 7.04012
7892508 21.6933
7892509 2497.51
7892510 18.378
7892511 7.41166
7892512 227.311
7892513 8.95717
7892514 896.107
7892515 358.5
7892516 12.1104
7892517 32.3309
7892518 3.44827
7892519 28.7963
7892520 481.558

Total number of rows: 257430

Table truncated, full table size 4002 Kbytes.

Supplementary file Size Download File type/resource
GSM1180558_6.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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