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Sample GSM1180557 Query DataSets for GSM1180557
Status Public on Jul 04, 2013
Title Bone marrow 122 clone LOW
Sample type RNA
Source name MSC clone after 28 days of culture, CD146Low
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
surface cd146: low
donor: 122
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur 122 LOW
Gene expression data from human cultivated bone marrow-derived MSC during 28 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
VALUE GC-RMA normalized signal intensity

Data table
7892501 67.8588
7892502 57.5963
7892503 19.7192
7892504 505.321
7892505 7.42124
7892506 26.9675
7892507 28.0792
7892508 10.9585
7892509 2151.95
7892510 33.3999
7892511 10.4244
7892512 577.911
7892513 20.3227
7892514 912.642
7892515 456.86
7892516 15.0233
7892517 33.7032
7892518 5.03514
7892519 24.2448
7892520 747.296

Total number of rows: 257430

Table truncated, full table size 4003 Kbytes.

Supplementary file Size Download File type/resource
GSM1180557_5.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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