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Sample GSM1180554 Query DataSets for GSM1180554
Status Public on Jul 04, 2013
Title Bone marrow 72 clone LOW
Sample type RNA
 
Source name MSC clone after 28 days of culture, CD146Low
Organism Homo sapiens
Characteristics cell type: cultivated bone marrow-derived mesenchymal stem cells
surface cd146: low
donor: 72
Treatment protocol No treatment.
Growth protocol MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
Extracted molecule total RNA
Extraction protocol Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
Label biotin
Label protocol Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
 
Hybridization protocol Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
Scan protocol GeneChip scanner 3000 and software AGCC.
Description MoPur72 LOW
Gene expression data from human cultivated bone marrow-derived MSC during 28 days.
Data processing Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
 
Submission date Jul 03, 2013
Last update date Jul 04, 2013
Contact name Nicolas Espagnolle
E-mail(s) nicolas.espagnolle@efs.sante.fr
Organization name STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
Street address Bâtiment INCERE 4 bis, avenue Hubert Curien
City Toulouse
ZIP/Postal code 31100
Country France
 
Platform ID GPL10739
Series (1)
GSE48540 CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment

Data table header descriptions
ID_REF
VALUE GC-RMA normalized signal intensity

Data table
ID_REF VALUE
7892501 50.8572
7892502 34.3204
7892503 8.86125
7892504 344.651
7892505 8.57211
7892506 30.2082
7892507 44.6751
7892508 12.1296
7892509 3204.61
7892510 4.75167
7892511 4.8164
7892512 401.512
7892513 7.02822
7892514 1494.83
7892515 347.099
7892516 14.1931
7892517 27.8978
7892518 4.74839
7892519 36.0414
7892520 535.992

Total number of rows: 257430

Table truncated, full table size 4003 Kbytes.




Supplementary file Size Download File type/resource
GSM1180554_2.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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