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Sample GSM1180041 Query DataSets for GSM1180041
Status Public on Dec 10, 2013
Title human radioresistant NPC, biological rep3 [miRNA]
Sample type RNA
Source name human radioresistant NPC cell line
Organism Homo sapiens
Characteristics cell line: radioresistance
Treatment protocol Radioresistant subclone of nasopharyngeal carcinoma CNE2-IR cell line was cultured and produced according to the experienment schedule to undergo five rounds of sublethal dose of irradiation (11 Gy),and the parent cell line CNE2 cell line sensitive to radiotherapy as the control.
Growth protocol Radioresistant NPC cell line CNE2-IR and its control cell line CNE2 were culture with 10 % FCS (Invitrogen, USA) and 1 % antibiotics in an incubator at 37 C with humidified 5 % CO2.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNAof 10^7 CNE2-IR and CNE2 cells was performed according to the manufacturer's instructions.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using Agilent miRNA Complete Labeling and Hybridization Kit according to the manufacturer's instructions, followed by .
Hybridization protocol 100 ng of Cy3 labelled RNA were hybridized to Human miRNA V2 Microarray 8 × 15K (G4470B, Agilent Technologies) according to manufacturer's instructions (miRNA Microarray System Protocol, Agilent Technologies). Arrays were scanned at 5 um resolution on an Agilent DNA Microarray Scanner (G2565BA, Agilent Technologies) using the default settings for miRNA Microarray v2.0 (miRNA Microarray System Protocol, Agilent Technologies).
Scan protocol Slides were scanned immediately after washing on the Agilent Feature Extraction(v10.7) using one color scan setting for array slides (Agilent Technologies Scanner G2505C).
Description miRNA expression data from radioresistant NPC cell line.
Data processing Images provided by the scanner were analyzed using Agilent's software Feature Extraction version10.7 using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
The raw data were normalized and analyzed by GeneSpring GX software version 7.3 (Percentile shift, normalizing to the 75th percentile)
Submission date Jul 03, 2013
Last update date Dec 10, 2013
Contact name Jia-Quan QU
Phone 13397319166
Organization name Central South University
Department Xiangya Hospital
Lab Key Laboratory of Cancer Proteomics of CMH
Street address Xiangya street No.87
City Changsha
State/province Hunan
ZIP/Postal code 410008
Country China
Platform ID GPL15019
Series (2)
GSE48502 MiRNA Expression data from human radioresistant and radiosensitive Nasopharyngeal Carcinoma Cells
GSE48503 Expression data from human radioresistant and radiosensitive Nasopharyngeal Carcinoma Cells

Supplementary file Size Download File type/resource
GSM1180041_CNE2IR-3.txt.gz 7.7 Mb (ftp)(http) TXT
Processed data are available on Series record

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