|
| Status |
Public on Dec 10, 2013 |
| Title |
human radioresistant NPC, biological rep2 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
human radioresistant NPC cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: radioresistance
|
| Treatment protocol |
Radioresistant subclone of nasopharyngeal carcinoma CNE2-IR cell line was cultured and produced according to the experienment schedule to undergo five rounds of sublethal dose of irradiation (11 Gy),and the parent cell line CNE2 cell line sensitive to radiotherapy as the control.
|
| Growth protocol |
Radioresistant NPC cell line CNE2-IR and its control cell line CNE2 were culture with 10 % FCS (Invitrogen, USA) and 1 % antibiotics in an incubator at 37 C with humidified 5 % CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNAof 10^7 CNE2-IR and CNE2 cells was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using Agilent miRNA Complete Labeling and Hybridization Kit according to the manufacturer's instructions, followed by .
|
| |
|
| Hybridization protocol |
100 ng of Cy3 labelled RNA were hybridized to Human miRNA V2 Microarray 8 × 15K (G4470B, Agilent Technologies) according to manufacturer's instructions (miRNA Microarray System Protocol, Agilent Technologies). Arrays were scanned at 5 um resolution on an Agilent DNA Microarray Scanner (G2565BA, Agilent Technologies) using the default settings for miRNA Microarray v2.0 (miRNA Microarray System Protocol, Agilent Technologies).
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent Feature Extraction(v10.7) using one color scan setting for array slides (Agilent Technologies Scanner G2505C).
|
| Description |
miRNA expression data from radioresistant NPC cell line.
|
| Data processing |
Images provided by the scanner were analyzed using Agilent's software Feature Extraction version10.7 using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
The raw data were normalized and analyzed by GeneSpring GX software version 7.3 (Percentile shift, normalizing to the 75th percentile)
|
| |
|
| Submission date |
Jul 03, 2013 |
| Last update date |
Dec 10, 2013 |
| Contact name |
Jia-Quan QU |
| E-mail(s) |
honpher@gmail.com
|
| Phone |
13397319166
|
| Organization name |
Central South University
|
| Department |
Xiangya Hospital
|
| Lab |
Key Laboratory of Cancer Proteomics of CMH
|
| Street address |
Xiangya street No.87
|
| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410008 |
| Country |
China |
| |
|
| Platform ID |
GPL15019 |
| Series (2) |
| GSE48502 |
MiRNA Expression data from human radioresistant and radiosensitive Nasopharyngeal Carcinoma Cells |
| GSE48503 |
Expression data from human radioresistant and radiosensitive Nasopharyngeal Carcinoma Cells |
|
