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Sample GSM1174470 Query DataSets for GSM1174470
Status Public on Jun 27, 2013
Title myelocytes
Sample type SRA
Source name myelocytes
Organism Mus musculus
Characteristics strain: C57BL/6J
tissue: bone marrow
age: 8-10 weeks
Extracted molecule total RNA
Extraction protocol Promyelocytes, myelocytes and granulocytes were purified using fluorescence activated cell sorting. RNA was extracted using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer IIx
Data processing Illumina Pipeline 1.5 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, then mapped to mm8 whole genome using bowtie v0.12.7
Alternative transcription start sites and gene expression were determined using Cufflinks Version 0.92.
Genome_build: mm9 & hg19
Supplementary_files_format_and_content: GTF file of transcripts
Submission date Jun 26, 2013
Last update date May 15, 2019
Contact name william ritchie
Organization name centenary institute
Street address Building 93
City Camperdown
State/province NSW
ZIP/Postal code 2050
Country Australia
Platform ID GPL11002
Series (2)
GSE48305 Orchestrated intron retention regulates normal granulocyte differentiation [RNA-Seq]
GSE48307 Orchestrated intron retention regulates normal granulocyte differentiation
BioSample SAMN02213906
SRA SRX315141

Supplementary file Size Download File type/resource
GSM1174470_C2.transcripts.gtf.bz2 3.5 Mb (ftp)(http) GTF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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