GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1173208 Query DataSets for GSM1173208
Status Public on Jun 22, 2016
Title Culture neuron -control electroporation 48h
Sample type SRA
Source name neurons electroporated with control plasmid
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
Stage: E16
tissue: brain
Treatment protocol Dissociated cells were electroporated with control plasmid or RNAi plasmid
Growth protocol Somatosensory cortexs of E16 rats' brain were dissected and dissociated into single cells. Medium contained Neurobasal medium, 10% FBS and 2% B27. Neurons were cultured for 48 hours
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Data processing Base-calling performed by using Illumina software
Removing 3’ adaptor sequence, empty reads, low quality Tags, Tags with a copy number of 1 and those Tags are too long or too short.
Sequenced reads were mapped to rn4 whole genome
Genome_build: rn4
Supplementary_files_format_and_content: text file containing gene expression
Submission date Jun 24, 2013
Last update date May 15, 2019
Contact name Pingping Zhao
Phone 8613671699349
Organization name Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Street address No. 320, YueYang Road, Shanghai, China
City Shanghai
ZIP/Postal code 200031
Country China
Platform ID GPL14844
Series (1)
GSE48236 Genome-wide analysis of rats' mRNA profile after knocking down PIWIL1 in embryonic cultured neurons.
BioSample SAMN02212505
SRA SRX314620

Supplementary file Size Download File type/resource
GSM1173208_Culture_neuron_-control_electroporation_48h-GeneExpression.txt.gz 1.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap