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Sample GSM1168995 Query DataSets for GSM1168995
Status Public on Jun 20, 2013
Title Uninfected PANC-1 cells rep3
Sample type RNA
Source name PANC-1 cells incubated in media
Organism Homo sapiens
Characteristics tissue: Pancreas/duct
morphology: epithlial
disease: epithelioid carcinoma
cell line: PANC-1
time: control
infection: control
Treatment protocol Cells were infected with GLV-1h153 at a multiplicity of infection of 5.0 and incubated at for zero, 6 and 24 hours.
Growth protocol PANC-1 cells (American type culture collection) were plated at 5 × 105 cells per well in 6-well plates and grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 1% antibiotic-antimycotic solution (Mediatech, Inc., Herndon, VA) and 10% fetal bovine serum (FBS) (Mediatech, Inc.) at 37oC under 5% CO2.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions (Qiagen Inc., Valencia, CA).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2002, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133A Array.
Scan protocol HG-U133A cDNA microarray chip images were scanned and processed to CEL files using the standard GCOS analysis suite (Affymetrix Inc).
Description PANC-1 cells cultured in media
Data processing CEL files were then normalized and processed to signal intensities using the gcRMA algorithm from the Bioconductor library for the R statistical programming system.
Submission date Jun 19, 2013
Last update date Jun 20, 2013
Contact name Dana Hadadd
Organization name Memorial Sloan-Kettering Cancer Center
Department Surgery
Lab Yuman Fong
Street address 410 E 69th st 4th floor
City New York
State/province NY
ZIP/Postal code 10019
Country USA
Platform ID GPL571
Series (1)
GSE48121 Expression data from human pancreatic cells PANC-1 infected with oncolytic vaccinia virus GLV-1h153

Data table header descriptions
VALUE log2 GCRMA signal

Data table
1007_s_at 9.84010850030058
1053_at 9.27215419015972
117_at 3.33308516810673
121_at 2.23612018808339
1255_g_at 2.14704910772043
1294_at 2.28334437070552
1316_at 2.14704910772043
1320_at 2.1481053909545
1405_i_at 8.42651061868968
1431_at 2.14704910772043
1438_at 2.14704910772043
1487_at 9.08823408577381
1494_f_at 2.14704910772043
1598_g_at 2.34724111643893
160020_at 2.163036667091
1729_at 7.61911167438814
177_at 2.46814455748279
1773_at 2.14716675376982
179_at 2.14704910772043
1861_at 5.30281345203243

Total number of rows: 22215

Table truncated, full table size 604 Kbytes.

Supplementary file Size Download File type/resource
GSM1168995_PANC-1_T0_3.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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