|
Status |
Public on May 14, 2014 |
Title |
ABRF-ILMN-N-BR-2 |
Sample type |
SRA |
|
|
Source name |
ABRF-ILMN-N-BR
|
Organism |
Homo sapiens |
Characteristics |
tissue: Brain reference rna: FirstChoice® Human Brain Reference Total RNA reference rna cat. #: #6050 reference rna lot #: 105P055201A
|
Extracted molecule |
total RNA |
Extraction protocol |
NA Rnase, RiboDep; exposure to 1 ng/ul RNase A for a sufficient time period to result in a RIN of 3, with the RNase then neutralized with 10 ul RNase Inhibitor (RiboLock EO0381, Thermo Scientific)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
Pure Human Brain Reference RNA Sample from Ambion, Inc.
|
Data processing |
STAR Aligner v2.3.0 Long Read counts were calculated using the Rmake pipeline (http://physiology.med.cornell.edu/faculty/mason/lab/r-make/) with Gencode v12 annotation. Genome_build: hg19 Supplementary_files_format_and_content: Counts table
|
|
|
Submission date |
Jun 17, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Christopher E Mason |
E-mail(s) |
chm2042@med.cornell.edu
|
Organization name |
Weill Cornell Medical College
|
Department |
Physiology and Biophysics
|
Lab |
Christopher Mason Lab
|
Street address |
1305 York Ave
|
City |
New York |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (2) |
GSE46876 |
The ABRF Next-Generation Sequencing Study (ABRF-NGS): Multi-platform and cross-methodological reproducibility of transcriptome profiling by RNA-seq. |
GSE48035 |
The ABRF Next-Generation Sequencing Study (ABRF-NGS): Multi-platform and cross-methodological reproducibility of transcriptome profiling by RNA-seq [Illumina HiSeq 2500] |
|
Relations |
BioSample |
SAMN02205276 |
SRA |
SRX307073 |