|
| Status |
Public on Jun 18, 2013 |
| Title |
STAV AB cell culture |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
STAV AB cell culture
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: mesothelioma cells
|
| Growth protocol |
Cells were grown in Gibco RPMI 1640 medium (Invitrogen) and 10% human AB serum, in 75cm2 tissue culture flasks (Sarstedt, Newton, USA) at 37 degrees C in 5% CO2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total genomic DNA was isolated using the GeneElute mammalian genomic DNA miniprep kit (Sigma-Aldrich, Germany)
|
| Label |
Cy3
|
| Label protocol |
2 μg of test DNA and 2 μg of reference DNA were differentially labelled by random priming using Cy3-dCTP (PA53021, GE Healthcare, Piscataway, NJ) and Cy5-dCTP (PA55021, GE Healthcare)
|
| |
|
| Channel 2 |
| Source name |
Pool of human peripheral blood DNA from 10 healthy female donors
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Pool of human peripheral blood DNA from 10 healthy female donors
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total genomic DNA was isolated using the GeneElute mammalian genomic DNA miniprep kit (Sigma-Aldrich, Germany)
|
| Label |
Cy5
|
| Label protocol |
2 μg of test DNA and 2 μg of reference DNA were differentially labelled by random priming using Cy3-dCTP (PA53021, GE Healthcare, Piscataway, NJ) and Cy5-dCTP (PA55021, GE Healthcare)
|
| |
|
| |
| Hybridization protocol |
Labelled samles were mixed with 100 μg of Cot-1 DNA (Roche, Basel, Switzerland) and hybridized to the array.
|
| Scan protocol |
Image acquisition was performed using the GenePix 4000B scanner (Axon Instruments Inc., Union City, CA).
|
| Data processing |
The average and coefficient of variation of fluorescence ratios for each measurement point were calculated. Data points displaying a coefficient of variation greater than 5% between at least two of the replica spots were discarded from further analysis.
|
| |
|
| Submission date |
Jun 17, 2013 |
| Last update date |
Jun 18, 2013 |
| Contact name |
Gustav Nilsonne |
| E-mail(s) |
gustav.nilsonne@ki.se
|
| Phone |
+46736798743
|
| Organization name |
Karolinska Institutet
|
| Street address |
Retzius väg 8 A2:3
|
| City |
Stockholm |
| State/province |
None/Not Applicable |
| ZIP/Postal code |
SE-17177 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17299 |
| Series (1) |
| GSE48019 |
Human STAV cells from AB and FCS sublines grown in cell culture or as xenografts |
|
