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Sample GSM1154188 Query DataSets for GSM1154188
Status Public on Sep 25, 2013
Title Identification of miR-143 target genes in PC3
Sample type RNA
 
Channel 1
Source name control miRNA transfected cell line
Organism Homo sapiens
Characteristics cell line: PC3
cell type: prostate cancer
transfection or treatment: control
Treatment protocol microRNA or siRNA transfection using Lipofectamine RNAiMAX (Invitrogen).
Growth protocol Normal cell culture medium RPMI 1640 or DMEM.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol following the manufacturer's instructions.
Label Cy3
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
 
Channel 2
Source name miR-143 transfected cell line
Organism Homo sapiens
Characteristics cell line: PC3
cell type: prostate cancer
transfection or treatment: miR-143
Treatment protocol microRNA or siRNA transfection using Lipofectamine RNAiMAX (Invitrogen).
Growth protocol Normal cell culture medium RPMI 1640 or DMEM.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol following the manufacturer's instructions.
Label Cy5
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
 
 
Hybridization protocol Agilent two-color gene expression hyb/wash protocol
Scan protocol Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution. Images were quantified using Agilent Feature Extraction software version 10.7.3.1
Data processing Agilent Feature Extraction Software version 10.7.3.1 was used for background subtraction and LOWESS normalization.
 
Submission date Jun 05, 2013
Last update date Apr 23, 2018
Contact name Naohiko Seki
E-mail(s) naoseki@faculty.chiba-u.jp
Phone +81-43-226-2971
Organization name Chiba University
Department Functional Genomics
Street address 1-8-1, Inohana
City Chiba
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL16699
Series (1)
GSE47657 Differentially expressed genes by anti tumor treatment in human cancer cell lines
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 4.860583815e-003
2 0.000000000e+000
3 0.000000000e+000
4 6.062138666e-002
5 0.000000000e+000
6 0.000000000e+000
7 -4.318286893e-002
8 2.501614149e-002
9 5.332309847e-002
10 -6.247385235e-002
11 4.675121270e-002
12 2.993438034e-003
13 5.391258728e-002
14 -8.762356868e-002
15 -1.891741690e-001
16 -6.428710174e-002
17 9.564594557e-002
18 6.679076391e-002
19 6.260312480e-003
20 1.223561081e-001

Total number of rows: 62976

Table truncated, full table size 1426 Kbytes.




Supplementary file Size Download File type/resource
GSM1154188_A2-PC3miR-143_PC3micont.txt.gz 20.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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