|
| Status |
Public on Dec 31, 2014 |
| Title |
Immortalized human bone marrow-derived mesenchymal stromal cell line DNA methylation [iMSC#3 (Culture I)] |
| Sample type |
genomic |
| |
|
| Source name |
iMSC#3 (Culture I)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: iMSC#3
sample origin: Human bone marrow-derived mesenchymal stromal cell
gender: Male
|
| Growth protocol |
Primary hMSCs were obtained from the hip of a healthy donor after informed consent. Mononuclear cells from the bone marrow were isolated using standard density centrifugation and cultured in minimum essential medium (MEM) Alpha medium (cat. no. 22561) supplemented with 20% FBS (PAA Laboratories), GlutaMAX, penicillin (100U/mL), and streptomycin (100 mg/mL) (all from Life Technologies) at 37C and 5% CO2. Non-adherent cells were removed after approximately 24 h by replacement of the medium. Subsequently, the adherent cells were allowed to expand until 70% confluence (5–6 days) before they were harvested and further expanded. iMSC#3 were grown under the same condition as hMSCs, but the medium was supplemented with 10% FBS.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega, Madison, USA).
|
| Label |
Biotin
|
| Label protocol |
Standard IIlumina label protocol
|
| |
|
| Hybridization protocol |
Standard IIlumina hyb protocol
|
| Scan protocol |
Standard IIlumina scan protocol
|
| Data processing |
Data extraction and initial quality control of the bead summary raw data were performed using GenomeStudio v2011.1 and the Methylation module v1.9, both provided by Illumina.
|
| |
|
| Submission date |
Jun 05, 2013 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Leonardo A. Meza-Zepeda |
| E-mail(s) |
L.A.Meza-Zepeda@ous-research.no
|
| Phone |
+47-99035706
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Tumor Biology
|
| Lab |
Meza-Zepeda
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE47649 |
Generation and Characterization of an immortalized human mesenchymal stromal cell line model [Illumina DNA methylation] |
| GSE47651 |
Generation and Characterization of an immortalized human mesenchymal stromal cell line model |
|
