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Sample GSM1154013 Query DataSets for GSM1154013
Status Public on Dec 31, 2014
Title Immortalized human bone marrow-derived mesenchymal stromal cell line DNA methylation [iMSC#3 (Culture I)]
Sample type genomic
Source name iMSC#3 (Culture I)
Organism Homo sapiens
Characteristics cell line: iMSC#3
sample origin: Human bone marrow-derived mesenchymal stromal cell
gender: Male
Growth protocol Primary hMSCs were obtained from the hip of a healthy donor after informed consent. Mononuclear cells from the bone marrow were isolated using standard density centrifugation and cultured in minimum essential medium (MEM) Alpha medium (cat. no. 22561) supplemented with 20% FBS (PAA Laboratories), GlutaMAX, penicillin (100U/mL), and streptomycin (100 mg/mL) (all from Life Technologies) at 37C and 5% CO2. Non-adherent cells were removed after approximately 24 h by replacement of the medium. Subsequently, the adherent cells were allowed to expand until 70% confluence (5–6 days) before they were harvested and further expanded. iMSC#3 were grown under the same condition as hMSCs, but the medium was supplemented with 10% FBS.
Extracted molecule genomic DNA
Extraction protocol DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega, Madison, USA).
Label Biotin
Label protocol Standard IIlumina label protocol
Hybridization protocol Standard IIlumina hyb protocol
Scan protocol Standard IIlumina scan protocol
Data processing Data extraction and initial quality control of the bead summary raw data were performed using GenomeStudio v2011.1 and the Methylation module v1.9, both provided by Illumina.
Submission date Jun 05, 2013
Last update date Dec 31, 2014
Contact name Leonardo A. Meza-Zepeda
Phone +47-99035706
Organization name Oslo University Hospital
Department Department of Tumor Biology
Lab Meza-Zepeda
Street address Ullernchausseen 70
City Oslo
ZIP/Postal code 0310
Country Norway
Platform ID GPL13534
Series (2)
GSE47649 Generation and Characterization of an immortalized human mesenchymal stromal cell line model [Illumina DNA methylation]
GSE47651 Generation and Characterization of an immortalized human mesenchymal stromal cell line model

Data table header descriptions
VALUE Normalized Average Beta using internal beadarray controls, no background substraction
iMSC#3 (Culture I).Detection Pval

Data table
ID_REF VALUE iMSC#3 (Culture I).Detection Pval
cg00000029 0.7273 0.0000
cg00000108 0.8834 0.0000
cg00000109 0.8750 0.0000
cg00000165 0.2421 0.0000
cg00000236 0.8477 0.0000
cg00000289 0.6451 0.0000
cg00000292 0.7136 0.0000
cg00000321 0.6473 0.0000
cg00000363 0.1626 0.0000
cg00000622 0.0162 0.0000
cg00000658 0.8055 0.0000
cg00000714 0.1470 0.0000
cg00000721 0.8764 0.0000
cg00000734 0.1034 0.0000
cg00000769 0.0461 0.0000
cg00000807 0.7514 0.0000
cg00000884 0.5380 0.0000
cg00000905 0.1016 0.0000
cg00000924 0.3766 0.0000
cg00000948 0.7558 0.0000

Total number of rows: 485577

Table truncated, full table size 11863 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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