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Sample GSM1154011 Query DataSets for GSM1154011
Status Public on Dec 31, 2014
Title Human none marrow-derived mesenchymal stromal primary culture (hMSC_Female)
Sample type RNA
 
Source name Human none marrow-derived mesenchymal stromal primary culture
Organism Homo sapiens
Characteristics cell type: hMSC
sample origin: Human bone marrow-derived mesenchymal stromal cell
gender: Female
Growth protocol Primary hMSCs were obtained from the hip of a healthy donor after informed consent. Mononuclear cells from the bone marrow were isolated using standard density centrifugation and cultured in minimum essential medium (MEM) Alpha medium (cat. no. 22561) supplemented with 20% FBS (PAA Laboratories), GlutaMAX, penicillin (100U/mL), and streptomycin (100 mg/mL) (all from Life Technologies) at 37C and 5% CO2. Non-adherent cells were removed after approximately 24 h by replacement of the medium. Subsequently, the adherent cells were allowed to expand until 70% confluence (5–6 days) before they were harvested and further expanded. iMSC#3 were grown under the same condition as hMSCs, but the medium was supplemented with 10% FBS.
Extracted molecule total RNA
Extraction protocol RNA was isolated using the standard TRIzol procedure (Invitrogen), and further purified with an RNeasy mini column (Qiagen GmbH, Hilden, Germany), according to the manufacturers’ instructions.
Label Biotin
Label protocol Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing The data was quantile normalisation in GenomeStudio (Illumina)
 
Submission date Jun 05, 2013
Last update date Dec 31, 2014
Contact name Leonardo A. Meza-Zepeda
E-mail(s) L.A.Meza-Zepeda@ous-research.no
Phone +47-99035706
Organization name Oslo University Hospital
Department Department of Tumor Biology
Lab Meza-Zepeda
Street address Ullernchausseen 70
City Oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL10558
Series (2)
GSE47648 Generation and Characterization of an immortalized human mesenchymal stromal cell line model [Illumina iMSC#3 and hMSC primary cultures]
GSE47651 Generation and Characterization of an immortalized human mesenchymal stromal cell line model

Data table header descriptions
ID_REF
VALUE Quantile normalised intensities in GenomeStudio (Illumina)
hMSC_Female.Detection Pval

Data table
ID_REF VALUE hMSC_Female.Detection Pval
ILMN_1343291 23853.1700 0.0000
ILMN_1343295 7258.5100 0.0000
ILMN_1651199 62.8044 0.7364
ILMN_1651209 74.0931 0.2169
ILMN_1651210 68.7817 0.4506
ILMN_1651221 69.4258 0.4221
ILMN_1651228 4597.9190 0.0000
ILMN_1651229 256.9223 0.0000
ILMN_1651230 75.1170 0.1753
ILMN_1651232 67.9774 0.4896
ILMN_1651235 84.7937 0.0299
ILMN_1651236 57.2541 0.9026
ILMN_1651237 145.6095 0.0000
ILMN_1651238 60.9610 0.7987
ILMN_1651249 66.8811 0.5455
ILMN_1651253 74.8246 0.1870
ILMN_1651254 2517.8970 0.0000
ILMN_1651259 91.9021 0.0039
ILMN_1651260 63.3610 0.7039
ILMN_1651262 5966.1290 0.0000

Total number of rows: 47318

Table truncated, full table size 1309 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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