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Sample GSM1153998 Query DataSets for GSM1153998
Status Public on Dec 31, 2014
Title IMSC#3, 0 hrs adipocyte differentiation, replicate 2
Sample type RNA
 
Source name Immortalized human bone marrow-derived mesenchymal stromal cell line IMSC#3, 0 hrs adipocyte differentiation, replicate 2
Organism Homo sapiens
Characteristics cell line: IMSC#3
sample origin: Human bone marrow-derived mesenchymal stromal cell
gender: Male
Growth protocol Cells were seeded in 48- and 6-well plates (Nunc) at 25,000 and 100,000 cells per well, respectively. Next day, the medium was replaced with adipocyte induction medium containing 1 µM Dexamethasone (Decadron), 0.5 mM iso-butyl-methyl-xanthine and 50 µM Indomethacine (both from Sigma-Aldrich). The differentiation medium was replaced twice a week for 21 days.
Extracted molecule total RNA
Extraction protocol RNA was isolated using the standard TRIzol procedure (Invitrogen), and further purified with an RNeasy mini column (Qiagen GmbH, Hilden, Germany), according to the manufacturers’ instructions.
Label Biotin
Label protocol Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing The data was quantile normalisation in GeneSpring GX10 (Agilent)
 
Submission date Jun 05, 2013
Last update date Dec 31, 2014
Contact name Leonardo A. Meza-Zepeda
E-mail(s) L.A.Meza-Zepeda@ous-research.no
Phone +47-99035706
Organization name Oslo University Hospital
Department Department of Tumor Biology
Lab Meza-Zepeda
Street address Ullernchausseen 70
City Oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL6884
Series (2)
GSE47647 Generation and Characterization of an immortalized human mesenchymal stromal cell line model [Illumina adipocyte differentiation]
GSE47651 Generation and Characterization of an immortalized human mesenchymal stromal cell line model

Data table header descriptions
ID_REF
VALUE Quantile normalised intensities in GeneSpring GX10 (Agielnt)
0h-2_F.Detection Pval

Data table
ID_REF VALUE 0h-2_F.Detection Pval
ILMN_1343291 217062.3525 0.0000
ILMN_1343295 82013.7395 0.0000
ILMN_1651199 312.0804 0.1871
ILMN_1651209 357.9437 0.0264
ILMN_1651210 284.2928 0.5428
ILMN_1651221 315.9757 0.1621
ILMN_1651228 9493.1687 0.0000
ILMN_1651229 903.5271 0.0000
ILMN_1651230 276.0078 0.6746
ILMN_1651232 442.8699 0.0079
ILMN_1651235 345.6030 0.0395
ILMN_1651236 332.8003 0.0725
ILMN_1651237 2833.1986 0.0000
ILMN_1651238 291.3527 0.4387
ILMN_1651249 268.6932 0.7773
ILMN_1651253 270.7375 0.7510
ILMN_1651254 9284.2780 0.0000
ILMN_1651259 304.3955 0.2701
ILMN_1651260 270.7375 0.7457
ILMN_1651262 31818.6058 0.0000

Total number of rows: 48803

Table truncated, full table size 1391 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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