|
| Status |
Public on Jun 30, 2013 |
| Title |
P21-3 |
| Sample type |
RNA |
| |
|
| Source name |
whole testis from postnatal day 21
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole testis
developmental stage: postnatal day 21
|
| Treatment protocol |
testes were freshly dissected out from embryonic or postnatal mice, snap freezed in liquid nitrogen and stored at -80 freezer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted using Trizol reagent and DNAase I treatment were performed to eliminate genomic DNA contamination, then mRNA was purified from total RNA after removal of rRNA
|
| Label |
Cy3
|
| Label protocol |
Each sample was amplified and transcribed into Cy3 fluorescent cRNA without 3' bias utilizing a random priming method
|
| |
|
| Hybridization protocol |
Each labeled cRNA was fragmented and heated at 60°C for 30min,50ul hybridization solution was assembled to the lncRNA expression microarray slide and incubated for 17 hours at 65°C in an Agilent Hybridization Oven.
|
| Scan protocol |
The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).
|
| Description |
lncRNAs and mRNAs expression
|
| Data processing |
Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Median normalization and subsequent data processing were performed with using the GeneSpring GX v12.0 software package (Agilent Technologies)
|
| |
|
| Submission date |
May 14, 2013 |
| Last update date |
Jun 30, 2013 |
| Contact name |
Nicole Ortogero |
| Organization name |
University of Nevada, Reno
|
| Department |
Physiology and Cell Biology
|
| Lab |
Dr. Wei Yan
|
| Street address |
1664 N. Virginia St
|
| City |
Reno |
| State/province |
Nevada |
| ZIP/Postal code |
89557 |
| Country |
USA |
| |
|
| Platform ID |
GPL15691 |
| Series (1) |
| GSE46896 |
Developmentally regulated lncRNAs expression in male germline |
|
