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Sample GSM1133658 Query DataSets for GSM1133658
Status Public on May 06, 2014
Title KO-RNAseq
Sample type SRA
Source name Primary Sertoli cell_control
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: testis
cell type: Primary Sertoli cell
genotype/variation: Wt1flox/flox (control)
Treatment protocol Wt1 was deleted after 4OH-tamoxifen treatment in Sertoli cells from Wt1-/flox;Cre-ERTM mice
Growth protocol Sertoli cells were growth in DMEM containing 10% fetal calf serum and incubated at 37°C and 5% CO2
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from Sertoli cells (Control and Wt1-KOT) using TRIzolH
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description Digital Gene Expression Tag Profiling
Data processing Illumina Casava1.7 used for basecalling
Filtered the adaptor sequence and PCR-duplication reads
Aligment to UCSC Mouse Genome mm9 by BWA 0.6.2-r126 with maximum 5 mismatch
Genome_build: mm9
Supplementary_files_format_and_content: Tab-delimited .txt files report statistics of gene annotation and expression for each sample.
Submission date May 06, 2013
Last update date May 15, 2019
Contact name wang xiaona
Organization name Institute of Zoology,Chinese Academy of Sciences
Department State Key Laboratory of Reproductive Biology
Street address Bei Chen Xi Lu #1
City Beijing
ZIP/Postal code 100101
Country China
Platform ID GPL13112
Series (1)
GSE46664 Digital Gene Expression Tag Profiling after Wt1 deletion in Sertoli cell
BioSample SAMN02117518
SRA SRX275430

Supplementary file Size Download File type/resource
GSM1133658_KO.GeneExpression.txt.gz 1.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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