|
| Status |
Public on May 06, 2014 |
| Title |
KO-RNAseq |
| Sample type |
SRA |
| |
|
| Source name |
Primary Sertoli cell_control
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: testis
cell type: Primary Sertoli cell
genotype/variation: Wt1flox/flox (control)
|
| Treatment protocol |
Wt1 was deleted after 4OH-tamoxifen treatment in Sertoli cells from Wt1-/flox;Cre-ERTM mice
|
| Growth protocol |
Sertoli cells were growth in DMEM containing 10% fetal calf serum and incubated at 37°C and 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Sertoli cells (Control and Wt1-KOT) using TRIzolH
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Digital Gene Expression Tag Profiling
|
| Data processing |
Illumina Casava1.7 used for basecalling
Filtered the adaptor sequence and PCR-duplication reads
Aligment to UCSC Mouse Genome mm9 by BWA 0.6.2-r126 with maximum 5 mismatch
Genome_build: mm9
Supplementary_files_format_and_content: Tab-delimited .txt files report statistics of gene annotation and expression for each sample.
|
| |
|
| Submission date |
May 06, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
wang xiaona |
| E-mail(s) |
xiaonayn@126.com
|
| Organization name |
Institute of Zoology,Chinese Academy of Sciences
|
| Department |
State Key Laboratory of Reproductive Biology
|
| Street address |
Bei Chen Xi Lu #1
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE46664 |
Digital Gene Expression Tag Profiling after Wt1 deletion in Sertoli cell |
|
| Relations |
| BioSample |
SAMN02117518 |
| SRA |
SRX275430 |
