|
Status |
Public on May 07, 2013 |
Title |
RA_SF_individual4 |
Sample type |
genomic |
|
|
Source name |
Rheumathoid Arthritis Synovial Fibroblast
|
Organism |
Homo sapiens |
Characteristics |
cell type: Synovial Fibroblast diagnosis: Rheumatoid arthritis
|
Treatment protocol |
no treatment
|
Growth protocol |
Fibroblast cultures were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Proteinase K extraction
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader (Illumina HiSeq2000) using standard recommended Illumina scanner setting
|
Description |
Rheumathoid Arthritis Synovial Fibroblast
|
Data processing |
Regular BeadStudio processing
|
|
|
Submission date |
May 06, 2013 |
Last update date |
May 07, 2013 |
Contact name |
Esteban Ballestar |
Organization name |
Josep Carreras Research Institute (IJC)
|
Lab |
Epigenetics and Immune Disease
|
Street address |
Ctra de Can Ruti, Camà de les Escoles s/n
|
City |
Badalona, Barcelona |
State/province |
N/A = Not Applicable |
ZIP/Postal code |
08916 |
Country |
Spain |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE46650 |
Identification of novel markers in rheumatoid arthritis through integrated analysis of DNA methylation and microRNA expression |
|